(1) de-novo synthesis and (2) synthesis by salvage pathways. sary for the synthesis of RNA, DNA, nucleotide-activated sugars, and lipids [3]. NUCLEOTIDE BIOSYNTHESIS Bio-synthesis of Purines & Pyrimidines e-mail: easybiologyclass@gmail.com mail@easybiologyclass.com www.easybiologyclass.com www.easybiologyclass.com II. However, as indicated above, the salvage pathway to thymidine nucleotide synthesis is especially important in the preparation for cell division. I. De-novo synthesis (synthesis from scratch): it is a biochemical pathway in which nucleotides are synthesized new from simple precursor molecules. Salvage pathway (recycle pathway): used to recover bases and nucleosides formed during the degradation of RNA and DNA Both purines and pyrimidines can be synthesized by 2 alterative pathways: the de novo pathways that metab-olize ribose and amino acids in a series of enzymatic reactions and the salvage pathways that A salvage pathway is a pathway in which a biological product is produced from intermediates in the degradative pathway of its own or a similar substance. The purine ring is synthesized along with the nucleotide i.e. recycling of the bases. APRT is inhibited by AMP. Split-thickness rabbit skins were minced and incubated in vitro with radioactive precursors selected to measure de nova and salvage pathways for pyrimidine nucleotide synthesis. HGPRT is the most important salvage enzyme. Classify each description as the de novo pathway, salvage pathway, or both pathways of pyrimidine nucleotide synthesis. Understand the Two Pathways of nucleotide biosynthesis (1) De-novo synthesis and (2) Salvage Pathways. Each category has at least one description. The salvage of pyrimidine bases has less clinical significance than that of the purines, owing to the solubility of the by-products of pyrimidine catabolism. Nucleotide Synthesis (De-novo and Salvage Pathways of Purine & Pyrimidine Biosynthesis PPT by easybiologyclass) 1. The pyrimidine synthesis is a similar process than that of Purines(Purines Synthesis).In the de novo synthesis of Pyrimidines, the ring is synthesized first and then it is attached to a ribose-phosphate to for a pyrimidine nucleotide.Pyrimidine rings are assembled from bicarbonate, aspartate, and Ammonia. This pathway supplies ribose sugar for the formation of the nucleotide. attached to the ribose sugar provided from HMP pathway. Uridine monophosphate, or UMP, is used as the example of the pyrimidine nucleotide. 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